LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1799..1895) /label=attL2 /note="attL2 (c)" misc_signal complement(1910..1929) /note="T7 Promoter/priming site (c)" primer_bind 1937..1953 /note="M13 Reverse priming site" gene 2066..2875 /note="Kanamycin resistance gene" rep_origin 2996..3669 /note="pUC origin" vector join(1807..3672,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1792 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:26499 IMAGE:4792498" /tissue_type="Brain, hypothalamus" /clone_lib="NIH_MGC_96" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 692..1792 /gene="B3GALT2" /gene_synonym=beta3Gal-T2 /gene_synonym=BETA3GALT2 /gene_synonym=GLCT2 /db_xref="GeneID:8707" /db_xref="HGNC:917" /db_xref="MIM:603018" CDS 692..1792 /dnas_title="UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2" /gene="B3GALT2" /gene_synonym=beta3Gal-T2 /gene_synonym=BETA3GALT2 /gene_synonym=GLCT2 /codon_start=1 /product="UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2" /protein_id="AAH22507.1" /db_xref="GI:21961261" /db_xref="GeneID:8707" /db_xref="HGNC:917" /db_xref="MIM:603018" /translation="MLQWRRRHCCFAKMTWNAKRSLFRTHLIGVLSLVFLFAMFLFFN HHDWLPGRAGFKENPVTYTFRGFRSTKSETNHSSLRNIWKETVPQTLRPQTATNSNNT DLSPQGVTGLENTLSANGSIYNEKGTGHPNSYHFKYIINEPEKCQEKSPFLILLIAAE PGQIEARRAIRQTWGNESLAPGIQITRIFLLGLSIKLNGYLQRAILEESRQYHDIIQQ EYLDTYYNLTIKTLMGMNWVATYCPHIPYVMKTDSDMFVNTEYLINKLLKPDLPPRHN YFTGYLMRGYAPNRNKDSKWYMPPDLYPSERYPVFCSGTGYVFSGDLAEKIFKVSLGI RRLHLEDVYVGICLAKLRIDPVPPPNEFVFNHWRVSYSSCKYSHLITSHQFQPSELIK YWNHLQQNKHNACANAAKEKAGRYRHRKLH" misc_feature 692..1792 /note="B3GALT2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caaagaaaac cctgtgacat acactttccg 721 aggatttcgg tcaacaaaaa gtgagacaaa ccacagctcc cttcggaaca tttggaaaga 781 aacagtccct caaaccctga ggcctcaaac agcaactaac tctaataaca cagacctgtc 841 accacaagga gttacaggcc tggagaatac acttagtgcc aatggaagta tttacaatga 901 aaaaggtact ggacatccaa attcttacca tttcaaatat attattaatg agcctgaaaa 961 atgccaagag aaaagtcctt ttttaatact actaatagct gcagagcctg gacaaataga 1021 agctagaaga gctattcggc aaacttgggg caatgaaagt ctagcacctg gtattcaaat 1081 cacaagaata tttttgttgg gcttaagtat taagctaaat ggctaccttc aacgtgcaat 1141 actggaagaa agcagacaat atcatgatat aattcaacag gaatacttag atacgtacta 1201 taatttgacc attaaaacac taatgggcat gaactgggtt gcaacatact gtccacatat 1261 tccatatgtt atgaaaactg acagtgacat gtttgtcaac actgaatatt taatcaataa 1321 gttactgaag ccagatctgc ctcccagaca taactatttc actggttacc taatgcgagg 1381 atatgcaccc aatcgaaaca aagatagcaa gtggtacatg ccaccagacc tctacccaag 1441 tgagcgttat cctgtcttct gttctggaac tggttatgtt ttttctggag atctggcaga 1501 aaagattttt aaagtttctt taggtatccg ccgtttgcac ttggaagatg tatatgtagg 1561 gatctgtctt gccaagttga gaattgatcc tgtaccccct cccaatgagt ttgtgttcaa 1621 tcactggcga gtctcttatt cgagctgtaa atacagccac ctaattacct ctcatcagtt 1681 ccagcctagt gaactgataa aatactggaa ccatttacaa caaaataagc acaatgcctg 1741 tgccaacgca gcaaaagaaa aggcaggcag gtatcgccac cgtaaactac atTAGGACCC 1801 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA 1861 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG 1921 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT 1981 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA 2041 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG 2101 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG 2161 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT 2221 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG 2281 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC 2341 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC 2401 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT 2461 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC 2521 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC 2581 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT 2641 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG 2701 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA 2761 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA 2821 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA 2881 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG 2941 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT 3001 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 3061 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 3121 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA 3181 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 3241 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 3301 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 3361 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA 3421 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 3481 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 3541 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 3601 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 3661 TGCTCACATG TT //